Cell Line Transfection Kits

Cell transfection refers to the technique of introducing exogenous molecules such as DNA, RNA, etc. into eukaryotic cells. With the continuous development of molecular biology and cell biology research, transfection has become a routine tool to study and control gene function in eukaryotic cells. It is used more and more widely in biological experiments such as studying gene function, regulating gene expression, mutation analysis and protein production. With the deepening of gene and protein function research, transfection has become a basic method often involved in laboratory work. Transfection can be roughly divided into three categories: physical mediation, chemical mediation and biological mediation. Electroporation, microinjection, and gene gun are examples of gene introduction into cells through physical methods; there are many chemically mediated methods, such as the classic calcium phosphate co-precipitation method, lipofection method, and various cationic substances mediated technology; biologically mediated methods, there are relatively primitive protoplast transfection, and various virus-mediated transfection techniques that are more common now.

Figure 1. Different paths to protein expression upon cells transfection with mRNA or pDNA.(Moradian H, et al.; 2020)

An ideal cell transfection method should have the advantages of high transfection efficiency and low cytotoxicity. Virus-mediated transfection technology is currently the method with the highest transfection efficiency and has the advantage of low cytotoxicity. However, the preparation procedure of the virus transfection method is complicated, and it often has strong selectivity for cell types, so it is difficult to popularize in general laboratories. Other physically and chemically mediated transfection methods each have their advantages and disadvantages. Therefore, more excellent exclusive transfection kits for different types of cells are needed to complete the most efficient cell transfection. To this end, CD BioSciences has developed exclusive cell transfection kits for different types of cells, through the precise design and modification of delivery carriers, as well as advanced technology platforms, can help you solve:

The challenges you might meet:

Common transfection reagents have low transfection efficiency
The cytotoxicity of the transfection vector is high
Transfection system with less load capacity
Inability to efficiently transfect under serum-containing conditions
High transfection efficiency of siRNA and plasmid DNA affects cell viability
Transfected cells are not suitable for single cell analysis
Some special cell types lack exclusive optimized transfection kits
Etc…

Key Features

Our Advantage:

CD BioSciences can provide a professional cell type-specific delivery system to achieve efficient cell transfection;
Biodegradable nanoparticle-based transfection agents—once inside cells, nanoparticles degrade into smaller, less toxic components, reducing cytotoxicity, facilitating transgene release, and increasing transfection efficiency
Efficient transfection can be performed in culture conditions up to 40% serum
Transfection kit includes transfection enhancer reagent
The system load is high, which can meet the transfection needs of a wide range of doses
Gentle enough for single-cell analysis
High transfection efficiency of siRNA and plasmid DNA without compromising cell viability
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
Professional design and service team to provide you with reliable service and technical support, timely feedback technical reports
Fluorescent carrier facilitates tracking and imaging of target molecules
Ready-to-use

Application candidates:

Can be used in various difficult-to-transfect cell lines;
It can be used in various transfection experiments that require high cell status;
More suitable as transfection for subsequent single-cell analysis;
For the transfection of cell lines that are particularly sensitive to carrier toxicity, you can choose this kind of exclusive cell line transfection kit
Requires transfection in serum-containing medium

CD BioSciences is focused on developing cell line transfection systems using our core technologies. With our high-quality products and services, your transfection effect will be greatly improved. If you can't find the perfect cell transfection system, you can contact us. We can provide one-to-one personal customization service.

Reference

Moradian H, et al.; mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification. Sci Rep. 2020, 10(1):4181.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.