Mouse Connective Tissue Cell MEF Transfection Kit

Modes of administration

Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Description

Mouse Embryonic Fibroblasts (MEF) are a connective tissue cell, procured from the embryos of mice, and oftentimes serve as the foundation for various types of research. With their ability to synthesize collagen and other extracellular matrix proteins, MEF cells have emerged as a highly valuable resource in studies concerning cellular senescence, aging, and the culture of other cell types. The MEF cell line, derived from murine (Mus musculus) embryonic fibroblast cells, is widely utilized as feeder cells for human embryonic stem cell research, as well as for studies related to mammalian embryonic tissue.

With numerous MEF cell lines exhibiting slightly different characteristics, they make an ideal transfection host in cell and molecular biology research. And, owing to their fibroblastic nature, MEF cells are relatively simple to identify when cultured in vitro.

MEF cells are not without their limitations, though. In particular, these cells tend to have a finite lifespan in culture, making it necessary to immortalize them through various means like virus infection and repeated transmission.

Nevertheless, Mouse Embryonic Fibroblasts (MEF) represent an incredibly useful model for the study of mammalian cells, and CD BioSciences is proud to offer nanoparticle-based transfection reagent kits that can be optimized based on specific project requirements.