Post-translational modification (PTM) is the process of adding specific modification groups to amino acid residues. PTM can alter the physicochemical properties of proteins, thereby affecting their folding, stability, active state, subcellular localization, and protein-protein interactions. It plays a crucial role in many cellular processes, such as cell differentiation, protein degradation, signal transduction, and metabolic regulation. PTM affects many aspects of plant cell biology and disease prevention. Therefore, identifying and quantifying PTM is crucial in the study of plant disease resistance mechanisms and crop variety improvement.

CD BioSciences provides quantitative plant PTM proteomics services based on the LC-MS/MS system. Quantify PTM proteomics through changes in molecular weight before and after protein modification. We provide a variety of PTM proteomics quantitative services to help customers gain a deeper understanding of the impact of PTM on plant protein functions and action mechanisms.

 Figure 1. Strategy for mapping post-translational modifications. (Mann M, Jensen ON. 2003)

Service Content

Quantitative Proteomics Service for Plant Acetylation

Our service can relatively quantify acetylation modifications in plant cell samples. Multiple biological samples can be analyzed simultaneously.

Quantitative Proteomics Service for Plant Phosphorylation

We combine advanced technology to compare multiple samples at once. We can also reduce the complexity of test samples and achieve ultra-precise quantification of phosphorylated proteins.

Quantitative Proteomics Service for Plant Glycosylation

We provide a quantitative method for glycosylated proteins applicable to any sample and allow for in vivo labeling. We use fewer biological samples to analyze complex quantitative data.

Quantitative Proteomics Service for Plant Methylation

We quantified methylated proteins through antibody enrichment, in vivo labeling, and tandem mass spectrometry. We can also compare the abundance of methylated peptides under different experimental conditions.

Quantitative Proteomics Service for Plant Ubiquitination

We utilize multiple affinity antibodies to enrich ubiquitinated peptide segments in complex samples and combine them with LC-MS/MS to achieve a large-scale quantitative analysis of plant ubiquitinated proteins.

Quantitative Proteomics Service for Plant SUMOylation

We enriched SUMOylated peptide segments through enzyme digestion and specific antibodies. Quantitative analysis of SUMOylated proteins in tissues, cells, and other plant samples using LC/MS.

Quantitative Plant Protein Disulfide Bond Service

We provide quantitative analysis services for disulfide bonds at the level of individual proteins and proteomics.

Application Fields

• Revealing the function of PTM proteins.
• Research of plant disease-related markers.
• Optimize drug design and develop more effective therapeutic drugs.
• Deeply understand the regulatory mechanisms and interactions of cellular signaling pathways.
• Research on the mechanism of cell metabolism regulation.
• Crop variety improvement.

Content of the Final Report

Experimental steps.

Related mass spectrometry parameters.

PTM site information.

Quantitative raw data.

Bioinformatics analysis, including protein coverage distribution, unique peptide distribution, protein GO functional classification, COG annotation, pathway annotation, and protein network interaction prediction.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

For research use only, not for clinical use.