Quantitative Plant Protein Disulfide Bond Service

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Quantitative Plant Protein Disulfide Bond Service

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Disulfide bonds are essential components in protein synthesis, participating in protein folding and stability. They are usually formed by the oxidation of thiol groups (- SH) and are easily destroyed by polar reagents, especially nucleophilic reagents. Moreover, the presence of incorrect disulfide bonds can lead to antibody aggregation. Therefore, studying disulfide bonds for protein structural characterization and functional analysis is of great significance.

CD BioSciences has established a protein disulfide bond analysis solution for individual proteins and at the omics level. Our service overcomes thein vitro exchange of protein disulfide bonds and maintains their natural structure. Our efficient MS analysis method helps clients reveal the effect of disulfide bonds on protein structure and function.   

Schematic diagram of sulfhydryl and S-S exchange reaction catalyzed by PDI. Figure 1. Schematic diagram of sulfhydryl and S-S exchange reaction catalyzed by PDI. (Fu J, et al. 2021)

Service Content Description
Quantitative Disulfide Bond Using Ellman's Reagent We can expose the thiol groups buried inside the protein using Ellman's reagent and quantify them using the absorbance of anions at 412nm. Our service can be widely used for the quantification of thiols or disulfide bonds in peptides and proteins. However, this method is not suitable for low-level protein detection.
Quantitative Cysteine Residues and Disulfide Bonds by LC/ESI-MS/MS We use biotin to label cysteine residues in proteins and denature them. Then analyze Cys or disulfide bonds through capillary LC/ESI-MS/MS. Our service provides a comprehensive analysis of cysteine and disulfide bonds in proteins or monoclonal antibodies and can reduce the occurrence of thiol-disulfide exchange reactions.

Application Fields

Our service is not limited to sample types or quantities and can be applied to protein disulfide bond analysis in multiple fields, including

  • Secondary or tertiary structure analysis of proteins.
  • Revealing protein stability.
  • Research on antibodies produced by the immune system.
  • Study the structure and function of various growth factors.
  • Optimizing the efficacy of protein drugs.
  • Protection mechanism during oxidation reaction.
Notification for Sample Preparation and Handling
Plant leaves, wet weight ≥ 200mg.
Plant roots, rhizomes, xylem, and phloem tissues ≥ 2g.
Algal tissue ≥ 5g.
Cell samples, cells ≥ 1×107.
Protein extract, concentration > 2mg/mL, total amount ≥ 1mg.
Please use sufficient dry ice for transportation and try to use faster shipping methods to reduce the possibility of sample degradation during transportation.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

Fu J, et al. (2021). PDI-Regulated Disulfide Bond Formation in Protein Folding and Biomolecular Assembly. Molecules. 26(1):171.

For research use only, not for clinical use.