Quantitative Proteomics Service for Plant Glycosylation

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Quantitative Proteomics Service for Plant Glycosylation

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Glycosylation is the process of connecting sugar chains with proteins to form glycosidic bonds under the action of glycosyltransferases. Glycosylation modification is widely present in plants, affecting the spatial conformation, activity, transportation, and localization of proteins.

CD BioSciences utilizes combinatorial mass spectrometry to analyze glycopeptides in complex glycoproteins and study the composition of the corresponding sugar chains. Our services help clients understand the function of glycoproteins in protein stability, signal transduction, and regulation of cell signaling pathways.

Detailed procedure for identification and quantification of proteins interacting with surface glycoproteins using multiplexed MS-based proteomics. Figure 1. Detailed procedure for identification and quantification of proteins interacting with surface glycoproteins using multiplexed MS-based proteomics. (Senhan Xu, et al. 2023)

Service Content Description
TMT/iTRAQ/DiLeu/IBT Service We provide an isometric heterotopic chemical labeling method TMT/iTRAQ/DiLeu/IBT suitable for any sample, which reacts with amines on glycopeptides. Our service reduces run-to-run variations, with low missing values, but does not allow in vivo labeling.
SILAC Service We use amino acids containing stable heavy isotopes for metabolic labeling when cultivating cells. Our service reduces system errors and allows for in vivo labeling, but is cost-effective for use with fewer biological samples.
MeSIL Service We offer MeSIL as a glycan marker, and we label the complete N-glycoprotein through a one-step reaction, which has high labeling efficiency and can be used for complex quantitative data analysis.
XIC Service We provide the XIC method to measure the intensity of glycopeptides during the runtime. Our service is available for any sample and there is no limit to the number of samples.

Application Fields

With years of research on glycosylated proteins, we are able to provide quantitative services in glycosylated proteomics to assist scientific research in various fields, such as

  • Plant disease-related marker investigation.
  • Plant disease mechanism research.
  • Systematic study of glycoprotein dynamics.
  • Study of protein-macromolecule interactions.
  • Studies on the regulation of plant immunity.
Notification for Sample Preparation and Handling
Plant tissue samples > 200mg.
Cell samples ≥ 1X107 cells.
Protein extract, total protein ≥ 100μg. Normal tissue and cell lysate can be used for protein extraction.
Please use sufficient dry ice for transportation and try to use faster shipping methods to reduce the possibility of sample degradation during transportation.

Other Services

We also provide the following bioinformatics services in protein post-translational modification analysis, such as

  • Functional annotation and enrichment analysis
  • Cluster analysis
  • Network analysis
  • Statistical analysis
  • Protein localization analysis

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

  1. Senhan Xu, et al. (2023). Deciphering the Properties and Functions of Glycoproteins Using Quantitative Proteomics.Journal of Proteome Research. 22 (6):1571-1588.

For research use only, not for clinical use.