Quantitative Proteomics Service for Plant Acetylation

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Quantitative Proteomics Service for Plant Acetylation

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Acetylation modification of proteins is the process of adding acetyl groups to lysine residues under the action of acetyltransferase. Acetylation affects plant gene expression, protein activity, and various physiological processes. It mainly controls the activation of transcription regulatory factors in the nucleus.

CD BioSciences provides quantitative plant acetylation proteomics services. We help clients reveal the transcriptional regulation and metabolic pathways of plant cells. We also provide ideas for customers to develop new drugs for treating plant diseases and pests.

Strategy for Proteomics Analysis of Lysine-Acetylated Proteins. Figure 1. Strategy for Proteomics Analysis of Lysine-Acetylated Proteins. (Kim SC, et al. 2006)

Service Content Description
SILAC Service We combine stable isotope labeling of cell samples with protein-modified antibody enrichment technology to relatively quantify acetylation modifications in plant cell samples.
iTRAQ/TMT Service We use tandem mass spectrometry with multiple stable isotope labels to label acetylated proteins in plant samples. We can perform relative quantification of acetylation modifications in 2-10 biological samples simultaneously.
Label Free Service We do not require stable isotope labeling reagents using the Label Free method, but the quantitative sample size for acetylation is limited.
Dimethyl Service We provide a Dimethyl method based on tandem mass spectrometry to quantify acetylated proteins. The combination of formaldehyde and sodium cyanide borohydride is used to label the peptide N-terminal and amino group of the lysine side chain.

Technical Advantages

Strict quality control system and ultra-high resolution detection system

Professional data preprocessing and analysis

High repeatability, specificity, and throughput

High resolution and sensitivity

Notification for Sample Preparation and Handling
Cells ≥ 1×108 cells/sample.
Young plant leaves, shoots ≥ 3g/sample.
Plant seeds, and fruits ≥ 5g/sample.
Plant rhizome, xylem, phloem: wet weight ≥ 5g/sample.
Protein extract, total protein ≥ 900μg, and concentration > 1μg/μL.
Store in liquid nitrogen or -80°C, transport with sufficient dry ice, and avoid repeated freezing and thawing. 

Application Fields

We provide a wide range of acetylation research techniques that can quantitatively acetylate modified proteins. We optimize the experimental plan according to the customer's requirements to provide comprehensive quantitative analysis in the field, such as

  • Post-translational modification studies of histones in epigenetics.
  • Gene expression regulation studies.
  • Apoptosis, cell metabolism, signaling research.
  • Protein stability studies.
  • Acetylation-induced metabolic disorders in plants.
  • Subcellular localization of regulatory proteins.
  • Enzyme activity regulation.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

  1. Kim SC, et al. (2006). Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. Mol Cel, 23(4):607-618.

For research use only, not for clinical use.