Quantitative Proteomics Service for Plant SUMOylation

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Quantitative Proteomics Service for Plant SUMOylation

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Small ubiquitin-like modifier (SUMO) refers to the covalent binding of ubiquitin-like proteins to other proteins under the action of enzymes. Unlike ubiquitination modification, SUMOylation does not cause protein degradation but rather participates in regulating biological processes, such as protein stability, nuclear-cytoplasmic transport, subcellular localization, and transcriptional regulation.

CD BioSciences enzymatically hydrolyzes protein samples and enriches modified peptide segments through enzyme digestion and specific antibodies. Using LC-MS/MS for in-depth and highly accurate quantitative analysis of SUMOylation proteins in tissues, cells, and other plant samples. 

Workflow for SUMO site recognition and proteome quantification. Figure 1. Workflow for SUMO site recognition and proteome quantification. (McManus FP, et al. 2018)

Service Content Description
LC-MS/MS Combined with Exogenous SUMOylation Proteomic Enrichment Service Our services include the enrichment of exogenous SUMOylated proteomes at both protein and peptide levels. We construct a cell line with stable expression and affinity enrichment tags. Our method has a higher enrichment efficiency for low-abundance SUMOylated proteins.
LC-MS/MS Combined with Endogenous SUMOylation Proteomic Enrichment Service We use strong anion exchange (SAX) chromatography to specifically enrich endogenous SUMO-modified substrates. Improve the quantitative efficiency of mass spectrometry for SUMOylated peptide segments. Our service can accurately reflect the true expression level of SUMO modification in organisms.

Our Service Workflow

Enzymatic hydrolysis of proteins in gel or solution.

Enrichment of SUMOylated proteins through specific antibodies.

HPLC separation followed by MS analysis.

Mass spectrometry data interpretation.

Notification for Sample Preparation and Handling
Fresh and tender tissues such as plant leaves, wet weight ≥ 25g.
Samples with high impurities or low protein content in plants, such as plant roots, rhizomes, xylem, and phloem tissues ≥ 210g.
Plant pollen ≥ 2400mg.
Algal tissue ≥ 25g.
Cell samples, cells ≥ 2×107.
Protein extract, concentration > 2mg/mL, total amount ≥ 1mg.
Please use sufficient dry ice for transportation to reduce sample degradation.

Application Fields

We have a highly sensitive HPLC-MS/MS platform that can analyze SUMOylation in various samples. Our services assist customers in researching SUMOylation proteins in multiple fields, such as

  • Research on protein nuclear-cytoplasmic transport.
  • Research on protein transcription activity.
  • Plant immune response.
  • Research on abiotic stress in plants.
  • Plant nutrient absorption.
  • Research on plant regulation of light morphogenesis.
  • Plant signal transduction regulation.
  • Plant sugar and energy metabolism.
  • Regulation of plant growth and development.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

  1. McManus FP, et al. (2018). Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9. Sci Rep. 8(1):7754.

For research use only, not for clinical use.