Plant Protein Properties Analysis Service
Protein is the material basis of life and one of the most important biomacromolecules in living organisms. All life requires proteins. To understand proteins, we must first understand the properties of proteins. In addition to using protein analysis software to predict various parameters, we verify and analyze the isoelectric point, stability hydrophobicity and purity of proteins through advanced instruments and methods. We carry out comprehensive analyses of proteins to help customers further research the function and mechanism of proteins.
CD BioSciences has instruments and equipment with high resolution and sensitivity. We master a variety of protein predictive analysis methods. Our services cover the determination of a variety of protein properties, providing customers with the most reliable and efficient analytical services.
Isoelectric point detection of plant proteins
| Method | plate method |
| capillary isoelectric focusing (cIEF) | |
| Protein sample requirement | dry powder or solution; protein content >200 μg |
| concentration >1 mg/ml | |
| purity >90% | |
| samples should be free of salt or low in salt | |
| Sample types | amino acids, peptides, recombinant proteins, enzymes and monoclonal antibodies. (If the sample is modified, please inform the type of modification in detail.) |
Stability detection of plant proteins
| Method | micro differential scanning fluorescence (nanoDSF) technology |
| pulse tracking technology | |
| Test index | denaturation temperature (Tm and Tonset) |
| denaturant concentration (Cm) | |
| folding free energy (ΔG and ΔΔG) | |
| starting temperature of protein aggregation (Tagg) | |
| Test projects | plant protein thermal stability |
| chemical stability | |
| isothermal stability | |
| half-life of protein | |
| protein denaturation | |
| renaturation ability | |
| Application field | protein design, optimization of protein purification conditions, formulation screening, buffer screening, protein quality control, identification of candidate biosimilars, verify biological drug functionality and long-term stability, etc. |
Hydrophobicity detection of plant proteins
| Method | ANS fluorescence probe method |
| CPA fluorescent probe method | |
| reverse high-performance liquid chromatography (HPLC) method | |
| SDS binding method | |
| surface tension method | |
| Protein sample requirement | the liquid protein concentration > 0.5mg/ml |
| not less than 500ul | |
| protein solids > 200ug | |
| Application field | identify the transmembrane region of membrane protein, analyze the secondary structure and spatial structure of globulin, determine whether the protein is easy to express, and apply it to food processing. |
Purity detection of plant proteins
| Method | SDS-PAGE |
| glutaral-free modified silver staining | |
| sedimentation rate measurement | |
| microfluidic diffusion fractionation (MDS) | |
| zinc reverse staining | |
| MALDI-TOF mass spectrometry | |
| light scattering method | |
| Sample types | antibodies, antigens, enzymes, growth factors, DNA-binding proteins, blood proteins, membrane proteins, etc. |
| Application field | increase the accuracy of downstream experiments, detect the purity of therapeutic protein APIs and drug products, and detect the degree of protein misfolding, irreversible aggregation, and degradation. |
Why Choose Us?
- We have accurate instruments and advanced methods to help our customers quickly analyze the properties of proteins.
- We have many protein properties testing items, which can be customized according to customer needs.
- We deliver the complete test report to our customers on time.
Related Services
Plant Protein Sequence Analysis Service
Plant Protein Advanced Structure Analysis Service
Plant Protein Modification Analysis Service
Plant Protein Localization Analysis Service
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
For research use only, not for clinical use.