Plant Protein Localization Analysis Service
Plant proteins are transported to the correct site to participate in the various life activities of the cell. Therefore, subcellular localization is a hot topic in protein function research and bioinformatics. The most common method is fluorescent protein labeling. The protein is expressed by gene transformation technology, the location of the target protein is observed, and its function is analyzed by software prediction or experimental verification.
CD BioSciences label the target protein with the fluorescent tag and use scanning confocal microscopy for image acquirement. We also offer simplified subcellular localization analysis and co-localization analysis with internal references (cell membrane, nucleus, and endoplasmic reticulum markers) to help clients gain a deeper understanding of the mechanisms of target proteins in signal transduction pathways and physiological regulation.
Figure 1. Chloroplast localization of ToxA62-132-GFP during transient expression in N. benthamiania leaves. (Sperschneider J, et al. 2017)
| Service Content | Service Description | |
| Fusion reporter gene localization | Plant overexpression vector construction | We provide fusion reporter gene methods that are widely used for protein subcellular localization. However, the strength of fusion protein expression can affect the localization results, especially for plasma membrane proteins. |
| Subcellular localization | ||
| Immunohistochemical localization | We use specific antibodies to detect the location of various antigens in vivo. In this method, specific antibodies for target proteins need to be easily obtained. | |
| Co-separated labeling enzyme-assisted localization | Our service can simultaneously detect the effectiveness of cell component separation. In this method, the protein to be tested requires a specific labeled enzyme that is easy to match. | |
| Proteomics localization | Proteins will be separated by bidirectional electrophoresis and identified by mass spectrometry to understand the abundance of proteins in different cellular locations. We can locate most high-purity proteins using this method. | |
Application Fields
- Characterize the cellular functions of hypothesized and newly discovered proteins.
- Reveal patterns of protein distribution in cells and understand complex pathways that regulate biological processes at the cellular level.
- Analyze the cellular function loss due to protein localization deviation.
- Study the mechanism of gene regulation, protein function, protein metabolism, and interactions.
Information That We Required
ORF of the target gene.
Gene name and other annotation information.
Deliverables
we can provide,
Sequencing results of the constructed site.
Constructed vector mapping, full sequence, and annotation information.
Images of protein subcellular localization.
Experimental report (experimental steps, reagents, and instrumentation).
Related Services
Plant Protein Sequence Analysis Service
Plant Protein Advanced Structure Analysis Service
Plant Protein Modification Analysis Service
Plant Protein Properties Analysis Service
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
Reference
- Sperschneider J, et al. (2017). LOCALIZER: subcellular localization prediction of both plant and effector proteins in the plant cell. Sci Rep. 7: 44598.
For research use only, not for clinical use.