Post-translational modification (PTM) is the chemical modification of a protein after translation. The biological properties of amino acid residues in proteins are changed by adding or removing specific groups. Protein modification regulates protein activity, localization, folding, function, and protein interactions. Protein modification plays an essential role in many cellular processes such as signal transduction, cell differentiation, cellular immunity and transcriptional regulation. The determination and analysis of protein modification is helpful for further analysis of protein function.

CD BioSciences uses liquid chromatography-mass spectrometry (LC-MS/MS) combined with database retrieval to identify modification sites including phosphorylation, acetylation, ubiquitination and glycosylation of plant proteins.

Figure 1. Protein modification analysis by top-down MS approach. (Zhang H, et al. 2011)

Service Content

Identification of plant protein modifications

Plant protein modification annotation

Quantitative analysis of plant protein modification

Validation of targeted protein modification sites

Differential expression analysis of plant protein modification

Establishment of plant protein modification database

Analysis of protein-modified expression profile

Assay Methods

LC-MS/MS, universal protein modification detection method

Radiometric kinase response, 32P-gamma-ATP is used to detect phosphorylation in vitro

Radioactive pulse labeling, detection of phosphorylation in vivo

Immunoprecipitation of chromatin (ChIP), can be used to detect acetylation and methylation of proteins

Western Blot, detection of protein ubiquitination in vivo and in vitro

Technical Route

Application Fields

• Plant protein phosphorylation modification, signal transduction, cell cycle, growth, and development.
• Plant protein glycosylation modification, protein folding, renewal and immune response.
• Plant protein acetylation modification, gene expression regulation, cell apoptosis, cell metabolism, protein stability, etc.
• Plant protein ubiquitin modification, cell cycle, apoptosis, transcriptional regulation, DNA repair and signal transduction.
• Plant protein methylation modification, chromatin structure, and transcriptional regulation.
• Plant protein propionylation, malonylation, glutarylation and succinylation modification, mainly exist in mitochondria and are closely related to metabolic processes.

Sample Requirement

Information for Customers

After the plant protein modification analysis, we will provide customers with,

Identification result statistics

Amino acid frequency distribution at modification sites

Secondary structure prediction of modification sites

Protein function analysis based on modification site

Go annotation analysis

Why Choose Us?

• Full scan mode, data independent acquisition mode, greatly reducing high-abundance interference, comprehensive acquisition of high-abundance signals.
• Multiple conventional retouching types can be scanned at once.
• Personalized targeted modification analysis can be performed according to the identified amino acid sites and modification types.

Related Services

Plant Protein Sequence Analysis Service

Plant Protein Advanced Structure Analysis Service

Plant Protein Properties Analysis Service

Plant Protein Localization Analysis Service

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

For research use only, not for clinical use.