Analyzing molecular interactions has been a classic topic in basic research and drug discovery. CD BioSciences, as a leading company in plant protein research, offers a range of plant protein-nucleic acid analyses, including plant protein screening for nucleic acid interactions, nucleic acid screening for plant protein interactions, protein-nucleic acid interaction validation, and identification of precise interaction sites.

Proteins, DNA, and RNA are the three members of the central law, and the study of protein-DNA/RNA interactions and regulation is an essential area of research in the post-genomic era and is of significance in the direction of epigenetics.

Service Content

Screening services of DNA interacting with plant proteins

With the development of molecular biology techniques, many essential genes have been isolated. The critical issue now is to reveal exactly how environmental factors and developmental signals control the transcriptional activity of genes. Therefore, we offer to identify and analyze DNA elements involved in the regulation of gene expression. Services we provide include

Screening service of RNA interacting with plant proteins

We provide RIP technology to screen RNA interacting with plant proteins. Our service can be used for gene expression analysis, marker detection, drug screening, and research on the mechanism of plant virus disease resistance.

Screening services of plant proteins interacting with nucleic acid

We provide services for screening and discovering plant proteins with known gene-specific binding. We can conduct high-throughput screening on the interaction network between known genes and plant transcription factors. Our services include

• DNA/RNA pull-down
• Plant yeast one-hybrid library construction and screening
• Chromatin isolation by RNA purification (CHIRP)-MS

Plant protein-nucleic acid interaction verification services

For the screened candidate interaction targets, we provide further interaction validation services, and our techniques include

• Electrophoretic mobility shift assay (EMSA)
• Yeast three-hybrid (Y3H)
• Plant protein and DNA/RNA co-localization (IF-FISH)

Plant protein-DNA interaction binding site assay service

By proving the interaction between two molecules, we can further explore the precise sites of protein-DNA interactions, enabling basic scientific research to achieve subtle observation angles. Our services are based on

Plant protein-RNA interaction binding site assay service

After validating plant protein-RNA interactions through a series of assays, we provide clients with precise identification of RBP binding sites at single-base resolution and single-cell level based on CLIP-seq and LACE-seq.

Application Fields

• Decipher the complex transcriptional regulatory networks involved in plant development, environment response, and stress tolerance.
• Uncover the mechanisms of epigenetic modifications, effects on gene expression, and action mechanisms in plant development and transgenerational inheritance.
• Genetically modify crops to improve yield, stress tolerance, nutrient content, and other desirable traits.
• The development of targeted genetic engineering strategies, gene editing technologies, and synthetic biology approaches can be used for crop improvement and the production of valuable plant-derived products.

CD BioSciences is dedicated to the analysis of plant protein-nucleic acid interactions. We have experienced botanical experts and cutting-edge equipment to help clients elucidate the molecular mechanisms of interactions and resolve plant protein functions. If you are interested in our services, please feel free to contact us! We will deepen your understanding of plant biology and pave the way for innovative strategies to improve crop productivity and sustainability.

For research use only, not for clinical use.