Plant Protein-DNA Interaction Binding Site Assay Service

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Plant Protein-DNA Interaction Binding Site Assay Service

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Exploring plant protein-DNA interaction binding sites is significant in plant biology research, revealing transcriptional regulation and promoter activity during plant development and stress response, and providing targets for genetic engineering and crop improvement. Therefore, CD BioSciences provides DNase I footprinting and methylation interference assay service to detect the precise sequence of plant protein-DNA binding to reveal more detailed action patterns.

Schematic of DNase I footprinting (left) and methylation interference assay (right). Figure 1. Schematic of DNase I footprinting (left) and methylation interference assay (right). (Song, C., et al., 2015, MacDonald, et al., 2007)

Service Content

DNase I footprinting assay service

Our DNase I footprinting technology utilizes proteins to protect DNA from enzymatic cleavage to detect DNA protein interactions. Our service combines fluorescence labeling with ABI sequencing, with a mature system, good repeatability, and high sensitivity.

  • Service flow

1) PCR amplifies and labels the region of interest.

2) Probe binds to the protein.

3) DNase I gradient digestion.

4) ABI sequencer assay.

Methylation interference assay service

We offer the methylation interference assay service which enables the investigation of the relationship between transcription factors and guanine residues at DNA binding sites. It is also a useful complement to DNase I footprinting assays for identifying the precise location of DNA-protein interactions.

  • Service flow

1) Preparation of radiolabeled DNA probes.

2) Methylation reactions.

3) Protein/DNA binding reaction.

4) Elution of radiolabeled DNA bands.

5) DNA fragmentation and denaturing PAGE analysis.

Notification for Sample Preparation and Handling

Type Details
Information
  1. Target DNA sample (PCR amplification product).
  2. PCR amplification primers.
  3. Purified specific proteins, and DNA-protein binding reaction conditions.
Samples
  1. PCR product concentration > 50ng/μL and total amount >50μL.
  2. PCR amplification primers for the target probe, the length of the target probe is 150-300bp.
  3. The total amount of protein is > 0.5mg, and the purity is > 90% (Protein expression and purification services can be provided by our company).

Deliverables

  • Detailed experimental procedure.
  • Experimental raw data.
  • Experimental images.
  • Complete experimental report.

CD BioSciences is a biological company specializing in plant proteins and is committed to providing clients with comprehensive plant protein-DNA interaction site assays. We help clients reveal protein function and infer the potential role of proteins in processes such as transcriptional activation, repression, chromatin remodeling, and DNA repair. If you are interested in our services, please feel free to contact us!

References

  1. Song, C., et al. (2015). Choosing a suitable method for the identification of replication origins in microbial genomes. Frontiers in microbiology. 6, 1049.
  2. MacDonald, et al. (2007). Characterization of a negative transcriptional element in the BRCA1 promoter. Breast Cancer Research. 9(4), R49.

For research use only, not for clinical use.