Plant Complex Proteins Expression and Purification Services
CD BioSciences is dedicated to the expression and purification of plant proteins, providing clients with strategies to increase the production of plant difficult-to-express proteins (DEPs). Our services include expression sequence optimization, host modification, molecular chaperone screening, efficient purification, and protein quality control.
Plant complex proteins, also known as DEPs, include toxins, membrane proteins (MPs), kinases, glycoproteins, and insoluble proteins. These proteins involve many physiological functions in plants, such as cell signaling, immune response, metabolism, etc., and are of great significance for plant pathology, medicine, and the pharmaceutical industry. However, they are difficult to express and purify in traditional expression systems.
Figure 1. Protocols for recombinant protein production and insoluble protein purification. (Ferrer-Miralles, N., et al., 2015)Service Content
Plant Glycosylated Protein Expression and Purification Service
Plant Disulfide Protein Expression and Purification Service
Plant Kinase Expression and Purification Service
Plant Toxic Protein Expression and Purification Service
Plant Membrane Protein Expression and Purification Service
Service Details
Codon optimization service
We use genetic engineering technology to provide clients with expression sequence adjustment services, such as optimization of mRNA secondary structure and Motif and other related parameters, to improve translation efficiency.
Expression vector construction service
We clone the target gene sequences into appropriate expression vectors for expression in host cells, including
- Insertion of the target gene.
- Selection of the appropriate promoter.
- Selection of appropriate tag or reporter gene.
Host cell transformation service
We select the appropriate expression system for clients and introduce the constructed vector into the host cells. Available expression systems include E. coli, yeast, mammalian cells, cell-free systems, or baculovirus-insect expression systems.
Culture and expression service
Protein expression of transformed host cells under appropriate culture conditions. We provide culture condition optimization service, including adjustment of medium components, temperature, pH, agitation speed, etc.
Purification and analysis service
We purify the target proteins from complex cell extracts by protein purification techniques such as
- Affinity chromatography
- Gel filtration chromatography
- Ion exchange chromatography
Then, we use various analytical techniques such as SDS-PAGE, Western blot, and mass spectrometry to identify and quantify proteins.
Application Fields
- Life science research.
- Drug Development.
- Diagnostic tool development.
- Industrial production.
- Protein structural biology research.
CD BioSciences is a leading biological services company specializing in the expression and purification of plant proteins. We provide cutting-edge solutions for clients seeking high-quality, customized protein production and purification services. If you are interested in our services, please contact us. Our highly skilled team can ensure the successful expression and purification of the most challenging proteins, providing unparalleled support for you.
References
- Saccardo, P., et al. (2016). Tools to cope with difficult-to-express proteins. Applied microbiology and biotechnology. 100(10):4347-4355.
- Ferrer-Miralles, N., et al. (2015). General introduction: recombinant protein production and purification of insoluble proteins. Methods in molecular biology (Clifton, N.J.). 1258:1–24.
For research use only, not for clinical use.