Plant Kinase Expression and Purification Service
Kinases in plants catalyze the transfer of phosphate groups from ATP to target molecules and play important roles in signal transduction, regulation of cellular processes, and plant growth and development. Expression and purification of kinases are key steps in the study of their structure, function, and regulatory mechanisms.
Figure 1. Protein kinase signaling networks in plant innate immunity. (Tena, G., et al., 2011)
CD BioSciences has extensive experience in plant protein research, providing customers with the expression and purification of plant kinases. Our services also include optimization of expression conditions, quality control, and evaluation of kinase activity to facilitate biochemical characterization of plant kinases.
Service Content
Expression system selection service
| Selectable expression systems | Details |
| Bacterial expression systems | Escherichia coli (E. coli), including BL21(DE3), BL21(DE3, pLysS) or BL21-CodonPlus(DE3)-RP Rosetta strains, such as Rosetta 2(DE3) |
| Yeast expression systems | Saccharomyces cerevisiae (S. cerevisiae), including BY4741, W303, PDR5 gene deletion mutant, BY4741 pdr5Δ Pichia pastoris, such as X-33 or GS115 |
| Plant expression systems | Nicotiana benthamiana Arabidopsis thaliana |
Targeted mutagenesis and plasmid construction service
We help our customers clone fragments encoding plant kinases into vectors, and then by targeted mutagenesis, we can rapidly and efficiently improve the traits and characterization of the target proteins.
We also provide rare codons (AGG, AUA, CUA, AGA, GGA, CCC, and CGG) or fusion tags (His tag, GST, MBP) to aid in kinase expression and purification.
Test expression service
We provide small-scale expression test prelabs to determine the appropriate inducer concentration and temperature for optimal activity and solubility of the target protein.
Protein expression and purification service
We offer our customers the service of transforming constructed plasmids into expression systems for large-scale protein expression and purification.
Our kinase purification service is based on
- Affinity chromatography
- Ion exchange chromatography
- Size exclusion chromatography
- Immobilized metal affinity chromatography (IMAC)
Quality control service
We implement strict quality control throughout the expression and purification process, including assessing protein expression levels and monitoring protein purity using SDS-PAGE or Western Blot.
Kinase assays service
- In vitro kinase assays using appropriate substrates and radiolabeled ATP.
- Fluorescence-based kinase activity assays.
- Antibody-based kinase activity assays.
| Types of plant kinases we can express and purify | |
| Receptor-like kinases (RLKs) | SnRK (SNF1-related protein kinases) |
| Calcium-dependent protein kinases (CDPKs) | Receptor-like cytoplasmic kinases (RLCKs) |
| Mitogen-activated protein kinases (MAPKs) | MAP kinase kinase kinases (MAP3Ks) |
| Cyclin-dependent kinases (CDKs) | Phosphatidylinositol 3-kinase (PI3Ks) |
| SnRK2 (Sucrose non-fermenting-1-related protein kinases 2) | |
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
References
- Tena, G., et al. (2011). Protein kinase signaling networks in plant innate immunity. Current opinion in plant biology. 14(5):519–529.
- Klein, S., et al. (2005). Expression and purification of active PKB kinase from Escherichia coli. Protein expression and purification. 41(1):162–169.
- Sonkoly, B., et al. (2011). Expression and purification of active protein kinases from wheat germ extracts. Methods in molecular biology (Clifton, N.J.). 77: 55–63.
For research use only, not for clinical use.