Glycosylation is an important modification of plant proteins, in which glycosyl groups form covalent glycosidic bonds with certain residues on proteins catalyzed by enzymes, thus affecting the physicochemical properties and biological functions of proteins. 

Figure 1. Preparation of glycosylated proteins in insect and mammalian expression systems. (Clarke, E. C., et al., 2017)

Glycosylated proteins play an essential role in the life processes of plants. CD BioSciences provides expression and purification services of plant glycosylated proteins to explore their functions in hormone signaling, cellular transport, development, growth, and potentially medicinal medicine.

Service Content

Glycoprotein sequence modification service

We provide our clients with services to alter and optimize the sequence of plant glycoproteins to aid in their expression and purification without affecting the function of the protein.

Expression system and vector selection service

We provide expression systems with the subcellular mechanisms required for post-translational modifications of glycosylation. We also analyze the effect of the plasmid vector backbone on protein expression levels and select vectors for transient or stable expression for our customers. Typically used for plant glycosylated protein expression include

• Insect Expression Systems
• Yeast expression systems
• Mammalian Expression Systems

Analysis service on the impact of temperature on glycoprotein expression

Most expressed glycoproteins form insoluble aggregates and fewer refold correctly. In the process of inducing protein expression, we screen the appropriate temperature for our customers to solve the glycoprotein aggregation problem.

Contaminant removal service

We can dissociate contaminants from possible protein-protein interactions based on

• Doubling the volume of wash buffer.
• Increasing the concentration of imidazole in the wash buffer.
• Increasing the concentration of imidazole in the sample.
• Adding urea to the sample.

Plant glycoproteins purification service

We provide our customers with glycoprotein purification methods based on the expression system, including

• Hydrophobic interaction chromatography
• Gel filtration chromatography
• Reversed-phase high-performance liquid chromatography (RP HPLC)
• Affinity chromatography with cross-linked dextran gels to capture glycoproteins
• Membrane separation

Protein purity verification service

After protein purification, we provide our customers with protein purity testing services based on colloidal blue staining, PAS (ThermoFisher Scientific, Waltham, MA), and protein blotting.

Glycan analysis service

We monitor specific glycan species or determine the relative amounts of glycans for our customers. Since glycans do not have chromophores, we offer 2-aminobenzamide (2-AB) labeled fluorescent labels to improve detection sensitivity. Our services include

• Analysis of N-linked glycans in glycoproteins
• Analysis of O-linked glycans in glycoproteins

Enzymatic glycan removal service

We offer our customers PNGase F and deglycosylation blends to remove glycans from purified glycoproteins.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details. 

For research use only, not for clinical use.