Recombinant Protein Expression Platform

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After years of exploration and development, CD BioSciences has established complete technology platforms for plant related protein expression. Our leading-edge technology and expertise accelerate the production of recombinant proteins, aiming to streamline the protein expression process from gene design and cloning to expression optimization and purification. With our platforms, researchers can efficiently generate high-quality proteins, such as plant functional recombinant proteins, plant structural proteins, and plant antibodies.

Our Recombinant Protein Expression Systems

Our platforms include a range of expression systems, providing flexibility in protein production based on specific requirements. Whether clients require high yields, post-translational modifications, or protein folding in the natural, our platforms will satisfy the request.

Prokaryotic expression system

Advantages	Clear genetic background multiplication, low cost, high expression 2H/13C/15N isotope labeling technology is stable and widely applicable.
Limitations	No transcriptional and post-translational processing functions, difficult to form correct spatial conformational folds. Expressed proteins are often insoluble and form inclusion bodies.

Applicable to simple eukaryotic proteins and isotope-labeled proteins.

Yeast expression system

Advantages	The expression process does not produce toxins. Processing of expression products can be carried out to maintain the activity and stability of the products. Expression product is secreted to extracellular, which is conducive to purification.
Limitations	Exogenously expressed proteins are easily degraded by their own secreted proteol. The fermentation cycle is relatively long and easy to be contaminated.

Suitable for expressing secreted proteins and proteins that are cytoplasmically localized and not modified by glycosylation.

Mammalian expression system

Advantages	Expression of glycosylated proteins and normal reconstitution of multiple subunits. High density and large culture volume.
Limitations	A long time is required to obtain highly expressed cell lines and high cost for large-scale culture.

Suitable for expression of secretory proteins, enzymes, transmembrane proteins ion channels, etc. The protein structure is close to the natural state.

Insect expression system

Advantages	Does not cause harm to humans or environmental pollution. Short vector construction period. Ideal for the expression of large segments of DNA. Can express multiple genes simultaneously in the same cell.
Limitations	Recombinant proteins are easily degraded.

Suitable for expression of secreted proteins and polyprotein mixtures.

Customized Recombinant Protein Expression Service

Figure 2. Recombinant protein expression systems. – CD BioSciences

Clients only need to provide the protein sequence or CDS, our experienced scientists can make a complete protein expression and purification solution in a short time.

Expression optimization

Our team employs cutting-edge bioinformatics analysis tools to aid in codon optimization and promoter selection to enhance protein expression and improve solubility.

Protein purification and characterization

After recombinant protein expression, we provide comprehensive protein purification and characterization strategies to ensure the production of highly pure and functional proteins.

Platform Features

One-stop service from gene design to protein preparation.
Self-developed a variety of high-expression vectors and strains.
Specialized R&D team for recombinant protein technology.
Provide high-purity protein customization service from mg to g.
Large-scale protein production platform.

CD BioSciences is a leader in recombinant protein expression. Our knowledgeable team works closely with clients to provide consultation on optimal expression systems, gene design, and project timelines to ensure success. If you are interested in our platforms or services, please contact us for more details.

For research use only, not for clinical use.