Plant Protein-Protein Interaction Analysis Services

CD BioSciences has been dedicated to the research of plant protein interactions for many years and has rich experience in the design and implementation of interaction projects. Whether it is basic biological research or agricultural crop improvement, we can provide clients with one-stop interaction research services from program flow design to technology implementation for studying plant protein interactions.

Figure 1. Schematic representation of plant-microbe protein interactions. (Nathalie Leborgne-Castel, & Bouhidel, K. 2014)

Service Content

Plant interaction protein screening service based on MS

We provide clients with screening services for plant-interacting proteins based on MS to further reveal protein functions and mechanisms regulating biological systems. Our services include

Plant yeast two-hybrid library construction and screening service

We offer plant yeast two-hybrid (Y2H) library construction and screening services to help clients explore protein-mediated signaling pathways and regulatory networks in plants. Our library construction services include

• Nucleoprotein yeast libraries
• Membrane protein yeast libraries
• Cytoplasmic protein yeast libraries

In addition, we provide Y2H point-to-point validation of the screened candidate target proteins.

Plant protein-protein interaction verification services

We offer various validation techniques to demonstrate protein-protein interactions to reveal the biological functions of candidate target proteins and identify action mechanisms at the molecular level. Our validation services include

• Co-immunoprecipitation (Co-IP)
• Bimolecular fluorescence complementation (BiFC)
• GST pull-down
• Fluorescence resonance energy transfer (FRET)
• Luciferase complementation imaging (LCI)
• Far-Western Blot

Plant protein-protein interaction affinity assay service

We provide clients with plant protein-protein interaction affinity assay service based on surface plasmon resonance (SPR) and biolayer interferometry (BLI) to evaluate the interaction strength between two proteins.

Application Fields

The study of plants' protein-protein interactions is significant in improving understanding of plant biology and has broad implications in various fields.

• Annotating the functional roles of proteins in plants provides valuable insights. Gain a deeper understanding of proteins' roles in cellular processes, signaling pathways, and regulatory networks.
• Uncover the complex network of proteins involved in plant responses to environmental stimuli, hormones, and stress. Contribute to deciphering the molecular mechanisms underlying plant growth, development, and adaptive responses.
• Construct regulatory networks that control gene expression and cellular responses, understanding how plants regulate growth and metabolism.
• It has practical applications in crop improvement and biotechnology. Improve crop productivity, disease resistance, stress tolerance, and nutritional quality, and contribute to the development of targeted breeding strategies for genetically engineered and improved crop varieties.

CD BioSciences has comprehensive expertise in protein-protein interactions. We provide valuable insights into the fundamental processes by which proteins regulate plant biology and help clients explore potential applications of plant proteins in agriculture and biotechnology. If you are interested in our services, please contact us for more details.

For research use only, not for clinical use.