Plant Protein-Protein Interaction Verification Services

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Plant Protein-Protein Interaction Verification Services

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Protein-protein interaction (PPI) forms the regulatory network that orchestrates plant cell function. CD BioSciences helps clients create plant PPI maps in complex cellular processes and at the systems biology level. We offer multiple methods to validate PPIs, understand the biological functions of unknown or known plant proteins, and identify action mechanisms at the cellular level.

Protein Interaction Verification Services.

Service Content

Co-immunoprecipitation (Co-IP) service

Advantages Limitations
  • Identifies unknown protein components of specific protein complexes.
  • Interacting protein complexes in the natural state can be isolated and obtained.
  • Low affinity and instantaneous protein interactions may not be detected.
  • The binding of two proteins may not be direct and false positives may occur.
  • Higher requirements for antibody selection.

Bimolecular fluorescence complementation (BiFC) service

Advantages Limitations
  • High sensitivity and intuitive visualization.
  • It is possible to analyze the localization and interaction intensity of proteins in different host cells such as animals, plants, and bacteria.
  • Various choices of fluorescent markers such as YFP, and RFP.
  • High requirements for temperature conditions.
  • Non-luminous fluorescent fragments may undergo spontaneous fusion and false positives.

GST Pull-Down service

Advantages Limitations
Direct detection of PPIs.
  • Only stable or strong interactions can be detected.
  • Protein interactions can be interfered with by endogenous proteins.

Fluorescence resonance energy transfer (FRET) service

Advantages Limitations
  • Suitable for all types of molecules in living and fixed cells.
  • Higher sensitivity and clearer imaging provide the most intuitive PPI localization and quantitative information.
Higher requirements for energy donor and acceptor spectra, alignments, quantum yields, extinction coefficients, water solubility, and anti-interference.

Luciferase complementation imaging (LCI) service

Advantages Limitations
  • Real-time, quantitative analysis in living cells can be realized.
  • It does not interfere with plant autofluorescence and lysis of cells to extract proteins.
  • Reflect the interaction status of proteins under plant physiological conditions.
The excessive size of fluorescent N-terminal and C-terminal fragments may interfere with the function or interactions of the fusion protein.

Far-Western Blot service

Advantages Limitations
  • Multiple tissue samples can be analyzed at once.
  • The molecular weights of interacting proteins can be determined immediately.
  • Weak interactions are poorly detected.
  • Interactions are difficult to detect if the target protein is low in the tissue.
  • Cannot detect interactions dependent on the natural structure.

Application Fields

  • Identify key players in signaling cascades, such as receptors, kinases, and transcription factors, and gain a deeper understanding of how plants respond to environmental stresses.
  • Uncover the roles of proteins in specific cellular processes, such as photosynthesis, hormone signaling, growth, and development.
  • Identify protein complex components and organization, revealing the roles and functional interdependencies.
  • Construct regulatory networks that control gene expression and cellular responses.
  • Crop improvement, understanding plant interactions with proteins related to disease resistance, yield, and stress tolerance, and developing transgenic crop varieties.

CD BioSciences offers services to validate plant protein-protein interaction. Our experienced researchers can customize experiments to meet clients' needs. If you are interested in our services, please feel free to contact us! We will unlock the secrets of plant protein-protein interaction and provide you with a deeper understanding of plant biology at the molecular level.

References

  1. Struk, S., et al. (2019). Exploring the protein-protein interaction landscape in plants. Plant, cell & environment. 42(2), 387-409.
  2. Zhang, Y., et al. (2010). Plant protein-protein interaction network and interactome. Current genomics. 11(1), 40-46.

For research use only, not for clinical use.