Plant Gene Editing Services

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Plant Gene Editing Services

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CD BioSciences provides plant genome editing technology based on sequence-specific nucleases (SSNs), such as zinc finger nucleases, transcription activator-like effector nucleases (TALEN), and CRISPR-Cas systems. Our services include plant genes knock out or knock-in, single base editing, and multiplex gene editing to improve a variety of superior traits such as crop yield or resistance to pests and diseases.

Genome editing technology, a newly developed and disruptive technology in life sciences, has been widely used in agriculture. Next-generation breeding technology based on genome editing technology can break the existing breeding bottleneck and shorten the breeding process, which brings unprecedented development opportunities for guaranteeing food security.

General procedure for plant genome editing.  Figure 1. General procedure for plant genome editing. (Gao C., 2021)

Service Content

Plant gene knock-in service

We provide complete and professional CRISPR Cas9-based plant knock-in solutions, customizing experimental protocols according to the client's project requirements and plant sample characteristics.

Plant gene knockout service

We offer clients a complete plant knockout strategy, including protocol design, knockout site screening, vector construction, transformation, and result validation analysis in a one-stop service.

Plant single base editing service

Our plant single-base editing is based on cutting-edge technologies such as cytosine base editor (CBE), adenine base editor (ABE), and prime editor (PE). Our service enables more effective and efficient editing at the single-base level.

Plant multiplex gene editing service

We offer the CRISPR Cas12a platform for plant multiplex gene editing, which can simultaneously target multiple genes at a given location. Our Cas12a system enables large-scale double allele editing through custom design and transformation, resulting in stable genetic modification of traits.

General service flow of plant gene editing

  1. Design appropriate nuclease based on the target sequence.
  2. Construct the genome editing vector.
  3. Verify the cutting activity of the nuclease by protoplasts.
  4. Introduce the genome editing system into plant cells.
  5. Genetic transformation of gene-edited cells by tissue culture of regenerated plants
  6. Screening and characterization of genotypes of genome-edited plants.

Our plant gene editing technology platform

Type zinc finger nucleases TALEN CRISPR-Cas
Advantages High efficiency, specificity Efficient, specific, and can target any DNA sequence. More efficient and specific, with strong multi-site editing capabilities.
Limitations Sequence-dependent, off-target effects, high cost. Plasmids construction is lengthy and homologous repair is time-consuming. Off-target effects, PAM limitation.

CD BioSciences is a leading biological company specializing in plant gene editing services. We provide cutting-edge solutions for precise and targeted genetic modification of plants. If you have any questions, please feel free to contact us, we prioritize innovation, accuracy, and efficiency to ensure the satisfaction of your specific experimental requirements.

References

  1. Gao C. (2021). Genome engineering for crop improvement and future agriculture. Cell, 184(6), 1621-1635.
  2. Patel, A., et al. (2023). Methods of crop improvement and applications towards fortifying food security. Frontiers in genome editing. 5, 1171969.
  3. Yanfei Mao, et al. (2019). Gene editing in plants: progress and challenges. National Science Review. 6, (3), 421-437.

For research use only, not for clinical use.