Plant Single Base Editing Service
CD BioSciences has a variety of single-base editing technologies, including cytosine base editor (CBE) for catalyzing C to T, adenine base editor (ABE) for catalyzing A to G, and prime editor (PE), etc. While achieving precise and efficient genome editing, it will minimize unnecessary by-products and cytotoxicity associated with double-stranded DNA breakage.
DNA single-base editing, a derivative of CRISPR/Cas9, enables targeted mutation of single nucleotides without cutting the DNA double-strand, opening the door to precision genome editing. This system is now widely used in agriculture, gene therapy, and crop breeding research.
Figure 1. Schematic of base editing, C to T base editing (a) A to G base editing (b). (Xie, Y., et al., 2022) Service Content
Plant cytosine base editing service
Our Cytosine Base Editor (CBE) fuses Cas9 or dCas9 with cytosine deaminases such as APOBEC. By targeting specific sites with gRNA, cytosine can be converted to uracil within a small editing window near the PAM site. We usually introduce a uracil DNA glycosylase inhibition (UGI) into the CBE technology to improve base editing efficiency.
Plant adenine base editing service
We offer the adenine base editor (ABE) platform, with the core building blocks nCas9 (D10A) and the adenine deaminase TadA, which allows direct translation of the plant's A/T base pairs to G/C base pairs. In addition, we offer services to improve the efficiency of ABE editing, including fusion NLS and the use of optimized codon sequences.
Comparison of CBE, ABE, and homologous recombination (HR)
| Type | CBE | ABE | HR |
| DNA cutting | SSB | SSB | DSB |
| Editing results | C-T | A-G | Stochastic |
| Product purity | >90% | >99% | 5%~50% |
| Efficiency | High | High | Low |
| Donor DNA | No | No | Yes |
| Cell cycle | Non-dependent | Non-dependent | Dependent |
| Genome rearrangement | No | No | Yes |
| Genome-wide off-targeting | Yes | No | No |
| RNA level off-targeting | Yes | Yes | No |
Other plant base editing services
- Prime editing services
We offer prime editing technology suitable for the mutagenesis of multiple bases within a defined sequence. We have fused reverse transcriptase (RT) and Cas9 notase proteins. By designing pegRNAs, we can induce subversion changes (A to C, T or G to C, T) in DNA sequences.
- STEME services
Our endogenous gene mutation base editor STEME fuses both cytosine deaminase and adenine deaminase at the N-terminus of nCas9, allowing editing of cytosine and adenine at the same target site.
- A&C-BEmax services
We provide an A&C-BEmax platform, which significantly reduces miss rate, widens the editing window, and increases editing efficiency through multiple rounds of optimization such as codons, nuclear localization signals, linker sequences, and UGI concatenation.
Base editing system optimization services
- Improve the purity of editing products, thus increasing editing efficiency.
- Reduce the incidence of Indels.
- Narrow the editing window and reduce neighboring site editing.
- Eliminate PAM sequence constraints.
- Capitalize on the dependence of base editing efficiency on sequence context.
Deliverables
Cell identification report.
Experimental report (including vector report, experimental procedure, sequencing raw data,etc.).
Single base mutant monoclonal pure cell line.
CD BioSciences offers plant base editing services with results you can trust. Once you place an order, our experienced scientists will work on your project through excellent platforms. We are waiting for your interesting project. For more information, feel free to contact us.
References
- Xie, Y., et al. (2022). Plant genome editing: CRISPR, base editing, prime editing, and beyond. Grassland Research.1(4), 234-243.
- Azameti, M. K., & Dauda, W. P. (2021). Base Editing in Plants: Applications, Challenges, and Future Prospects. Frontiers in plant science. 12, 664997.
- Liu, H., et al. (2023). Precise genome editing with base editors. Medical review (Berlin, Germany). 3(1), 75-84.
For research use only, not for clinical use.