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XRCC5 (+/-) Cell Line
SPL-04024
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| XRCC5 (+/-) |
| Target Information | |
|---|---|
| Target Name | XRCC5 |
| Gene Abbr. | XRCC5 |
| Gene ID | 7520 |
| Full Name | X-ray repair cross complementing 5 |
| Alias | KARP-1, KARP1, KU80, KUB2, Ku86 |
| Species | Human |
| Genomic Locus | 2q35 |
| Introduction | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | NALM-6 |
| Genotype | XRCC5 (+/-) |
| Description | XRCC5 (+/-) |
| Parental ATCC ID | DSMZ: ACC 128 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 8,11; D16S539: 12,13; D5S818: 13; D7S820: 10,12; THO1: 7,9.3; TPOX: 8,11; vWA: 18,19; ATCC |
| Disease | Acute lymphoblastic leukemia |
| Tissue | B-Cell |
| Gender | Male |
| Key Endogenous Mutations | CDKN2A, CDKN2a(p14), MSH2, NRAS |
| CVT Selection Condition | G418 resistant - 1 mg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Freeze Medium | Complete growth media 40% + USDA approved FBS 50% + DMSO 10% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.