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XRCC4 (+/-) Cell Line
SPL-04022
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| XRCC4 (+/-) |
| Target Information | |
|---|---|
| Target Name | XRCC4 |
| Gene Abbr. | XRCC4 |
| Gene ID | 7518 |
| Full Name | X-ray repair cross complementing 4 |
| Alias | SSMED |
| Species | Human |
| Genomic Locus | 5q14.2 |
| Introduction | The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015]. |
| Product Details | |
|---|---|
| Cell Line Model | hTERT RPE-1 |
| Genotype | XRCC4 (+/-) |
| Description | XRCC4 (+/-) |
| Parental ATCC ID | CRL-4000 |
| STR Profile | Amelogenin: X; CSF1PO: 12,14; D13S317: 11,12; D16S539: 11; D5S818: 11; D7S820: 10,11; THO1: 9; TPOX: 8; vWA: 17, 18; ATCC |
| Disease | (Normal) |
| Tissue | Retina pigment epithelium |
| Gender | Female |
| Key Endogenous Mutations | RB1 |
| CVT Selection Condition | Hygromycin resistant - 0.01 mg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:4-1:8. |
| Culture Medium | DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:4-1:8. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.