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USP30 Knockout Cell Line
SPL-03906
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 1bp deletion |
| Target Information | |
|---|---|
| Target Name | USP30 |
| Gene Abbr. | USP30 |
| Gene ID | 84749 |
| Full Name | ubiquitin specific peptidase 30 |
| Species | Human |
| Genomic Locus | chr12:109052701 |
| Transcript | NM_032663 |
| WT Expression Level | 13.74 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM, Dec 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 1bp deletion in a coding exon of USP30. |
| Description | 1bp deletion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | CGGCGATGACCGCGGCCGAC |
| PCR Primer |
Forward: CCTGTTGCTAAGGGAAAAGAAAGAA Reverse: GACCCCAACTAGGGCATGAAAAAG |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.