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TRAF2 Antibody
SPA-11210
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TRAF2 |
| Gene Abbr. | TRAF2 |
| Gene ID | 7186 |
| Full Name | TNF receptor associated factor 2 |
| Alias | MGC:45012, RNF117, TRAP, TRAP3 |
| Introduction | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses. Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.TRAF2 is phosphorylated at Ser11 by IKK-ε, promoting K63-linked ubiquitination, NF-κB activation, and cellular transformation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to amino acids 6-31 (VTPPGSLELLQPGFSKTLLGTKLEAK) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 92% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1) NP_066961.2 = 501 aa. 2) CAI12705.1 =121 aa. 3) CAI12704.1 = 422 aa. 4) CAI15104.1 = 193 aa. 5) BAD96681.1 = 205 aa. |
| Usage | |
|---|---|
| Application | WB, FC, IF, IHC, IP |
| Dilutions | Western Blot (1:1000-1:2000); Immunofluorescence (1:10-1:500); Immunohistochemistry (1:10-1:500); Immunoprecipitation (1:50-1:200) |
| MW(KDa) | 53 |
| Reactivity | Human |
| Specificity | The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by Western Blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Whole antisera. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.