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Tpl2/MAP3K8 Antibody
SPA-11178
| Size | Price |
| 100 µg | Online Inquiry |
| 50 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Tpl2 |
| Gene Abbr. | MAP3K8 |
| Gene ID | 1326 |
| Full Name | mitogen-activated protein kinase kinase kinase 8 |
| Alias | AURA2, COT, EST, ESTF, MEKK8 |
| Introduction | Tpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine/threonine kinase expressed primarily in hematopoietic tissues, lung and liver. Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5. Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1. Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription. Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthetic peptides corresponding to unique epitope on MAP3K8. The peptide sequence was selected from C-terminal-region, amimo acids 365-467. This peptide was covalently modified post-synthetically to achieve desired antigenicity. |
| Usage | |
|---|---|
| Application | WB, ELISA, IHC, IP |
| Dilutions | Western Blot (> 1:500); ELISA (< 1:10000); Immunohistochemistry (1:50-1:150); Immunoprecipitation (1:200) |
| MW(KDa) | 60, 62 |
| Reactivity | Human, Mouse, Rat |
| Specificity | This antibody detects a single band of approximately 57kDa in PC-MAP3K8 samples. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris/Glycine buffer, pH 7.4-7.8, HEPES, BSA 0.5%, glycerol 30% . |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.