Total Notch1ELISA Kit - CD BioSciences

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Total Notch1ELISA Kit

Total Notch1ELISA Kit

SPK-00871

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Target Information
Target Name Notch1
Gene Abbr. NOTCH1
Gene ID 4851
Full Name notch receptor 1
Alias AOS5, AOVD1, TAN1, hN1
Introduction Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes.The NICD of murine Notch1 is released (activated) by cleavage between Gly1743 and Val1744 (corresponding to Gly1753/Val1754 in human Notch1). Mutations that result in constitutive activation of Notch1 are associated with many different cancers, including a majority of cases of T cell acute lymphoblastic leukemia (T-ALL). Activation may be due to mutations in Notch1 itself, or in components of the ubiquitin ligase complex that negatively regulates the Notch signaling pathway.
Usage
Application ELISA
Reactivity
Sensitivity 19.5 pg/mL
Detection Range 78 pg/mL - 5000 pg/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.