Total NF-κB p65 Sandwich ELISA Kit - CD BioSciences

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Total NF-κB p65 Sandwich ELISA Kit

Total NF-κB p65 Sandwich ELISA Kit

SPK-00852

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Target Information
Target Name NF-κB
Gene Abbr. RELA
Gene ID 5970
Full Name RELA proto-oncogene, NF-kB subunit
Alias CMCU, NFKB3, p65
Introduction Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.NF-κB assembly with IκB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310. This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-κB.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Sensitivity This kit detects endogenous levels of NF-kappaB p65 enzyme.

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