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TOP2B (-/-) Cell Line
SPL-03681
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| TOP2B (-/-) |
| Target Information | |
|---|---|
| Target Name | TOP2B |
| Gene Abbr. | TOP2B |
| Gene ID | 7155 |
| Full Name | DNA topoisomerase II beta |
| Alias | TOPIIB, top2beta |
| Species | Human |
| Genomic Locus | 3p24 |
| Introduction | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]. |
| Product Details | |
|---|---|
| Cell Line Model | NALM-6 |
| Genotype | TOP2B (-/-) |
| Description | TOP2B (-/-) |
| Parental ATCC ID | DSMZ: ACC 128 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 8,11; D16S539: 12,13; D5S818: 13; D7S820: 10,12; THO1: 7,9.3; TPOX: 8,11; vWA: 18,19; ATCC |
| Disease | Acute lymphoblastic leukemia |
| Tissue | B-Cell |
| Gender | Male |
| Key Endogenous Mutations | CDKN2A, CDKN2a(p14), MSH2, NRAS |
| CVT Selection Condition | No antibiotic resistance |
| Cell Number | 5x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Freeze Medium | Complete growth media 40% + USDA approved FBS 50% + DMSO 10% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.