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TNF-α Antibody
SPA-11045
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TNF-α |
| Gene Abbr. | TNF |
| Gene ID | 7124 |
| Full Name | tumor necrosis factor |
| Alias | DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F |
| Introduction | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT). Leu80-Leu235. P06804. |
| Usage | |
|---|---|
| Application | WB, ELISA, FC, IF |
| Dilutions | Western Blot (2 µg/mL); ELISA Capture (0.2-0.8 µg/mL); ELISA Detection (0.1-0.4 µg/mL); Immunofluorescence (5-15 µg/mL); Flow Cytometry (2.5 µg/10^6 cells); Neutralization (0.1-0.4 µg/mL) |
| MW(KDa) | 17 (mature), 25 (precursor) |
| Reactivity | Human, Mouse |
| Specificity | Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western Blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.