Online Inquiry
Tie-2 Antibody
SPA-10757
| Size | Price |
| 0.025 mL | Online Inquiry |
| 0.1 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Tie2 |
| Gene Abbr. | TEK |
| Gene ID | 7010 |
| Full Name | TEK receptor tyrosine kinase |
| Alias | CD202B, GLC3E, TIE-2, TIE2, VMCM |
| Introduction | Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis. A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation. Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | MM0018-21G7 |
| Isotype | IgG1 |
| Immunogen | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human extracellular domain of Tie2 fragment. |
| Usage | |
|---|---|
| Application | WB, ELISA, IHC |
| Dilutions | Western Blot (1:500-1:1000); ELISA (1:200-1:400); Immunohistochemistry (1:50) |
| MW(KDa) | 160 |
| Reactivity | Human |
| Specificity | Recognizes human Tie2, no cross-reactivity with human Tie1. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from 0.2 µm filtered PBS. |
| Preservative | No Preservative |
| Storage Temp. | Aliquot and store at -20 °C or -80 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.