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Th cDNA ORF Clone, Mouse, In cloning vector
SPD-15354
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| Full length Clone DNA of Mouse tyrosine hydroxylase. |
| Target Information | |
|---|---|
| Species | Mouse |
| Target Name | Tyrosine Hydroxylase |
| Gene Abbr. | Th |
| Gene ID | 21823 |
| Full Name | tyrosine hydroxylase |
| Introduction | Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms. This enzyme is required for embryonic development since TH knockout mice die before or at birth. Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH. Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels. |
| Product Details | |
|---|---|
| Description | Full length Clone DNA of Mouse tyrosine hydroxylase. |
| NCBI Ref Seq | NM_009377.1 |
| RefSeq ORF Size | 1497 bp |
| Sequence Information | Identical with the Gene Bank Ref. ID sequence except for the point mutation 833 A/G resulting in the amino acid his substitution by arg. |
| Vector | pGEM-T Vector |
| Quality Control | The plasmid is confirmed by full-length sequencing. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
| Usage | |
|---|---|
| Sequencing Primers | SP6 and T7 or M13-47 and RV-M |
| Antibiotic in E.coli | Ampicillin |
| Shipping | Each tube contains lyophilized plasmid. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.