Online Inquiry
TGF-β 1 Antibody
SPA-10685
| Size | Price |
| 100 µL | Online Inquiry |
| 20 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TGF-β |
| Gene Abbr. | TGFB1 |
| Gene ID | 7040 |
| Full Name | transforming growth factor beta 1 |
| Alias | CED, DPD1, IBDIMDE, LAP, TGF-beta1 |
| Introduction | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis. TGF-β elicits signaling through three cell surface receptors: type I type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases. The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription.There are three TGF-beta family members, designated TGF-β1, TGF-β2, and TGF-β3, which are encoded by distinct genes and are expressed in a tissue specific manner. TGF-β proteins are synthesized as precursor proteins that are cleaved and reassembled in association with other proteins to form latent complexes. Activation occurs by proteolytic release of TGF-β monomers, which dimerize to form the mature TGF-β ligands. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthetic peptide from human TGF-beta 1. |
| Usage | |
|---|---|
| Application | WB, ELISA, IF, IHC |
| Dilutions | Western Blot (1:100-1:500); ELISA (1:10000-1:20000); Immunofluorescence (1:10-1:500); Immunohistochemistry (5 µg/mL) |
| MW(KDa) | 12 |
| Reactivity | Human, Mouse, Equine |
| Specificity | A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFb1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 20 mM Potassium Phosphate (pH 7.2) and 0.15 M NaCl. |
| Preservative | 0.01% Sodium Azide |
| Storage Temp. | Keep as concentrated solution. Aliquot and store at -20 °C or below. |
| Handling | Avoid multiple freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.