RBL-2H3 Kit-Rat Basophilic Leukemia Cells

Modes of administration

Electroporation Efficiency:Electroporation kit provides 80-95% delivery efficiency of siRNA. Electroporation efficiency was determined by RT-PCR.

Description

Basophilic leukemia cells from a Wistar Rat (Rattus norvegicus) were used to create RBL-2H3 in the National Institute of Dental Research's Laboratory of Immunology in 1978. The ability of these cells to release mediators like histamine makes them helpful. They have benefited research on mast cell secretion. Even though almost all fetal bovine serum lots promote the formation of these cells, some lots' cells degranulate more effectively after FcERI aggregation. Another non-degranulating basophilic leukemia cell line is RBL-1 (CRL-1378). Research on allergies, inflammation, and mast cell biology frequently uses the cell line RBL-2H3. These cells are frequently utilized as a model for researching mast cell activity and allergy reactions because they were extracted from a rat basophilic leukemia (RBL) tumor. Numerous traits of RBL-2H3 cells make them useful in study. IgE receptors (FcRI) are highly expressed on the surface of these cells, enabling them to react to allergens and initiate mast cell degranulation. Histamine, leukotrienes, and interleukins are just a few of the mediators and cytokines that RBL-2H3 cells secrete that are crucial to the allergic reaction. The mechanisms underpinning mast cell activation and degranulation, the function of particular chemicals and pathways in allergic responses, and the testing of novel treatments for allergic illnesses are all studied by researchers using RBL-2H3 cells. RBL-2H3 cells are a crucial tool for expanding our knowledge of mast cell biology and allergy, in general.