POLYMER Transfection Kit

Modes of administration

Systemic intravenous (i.v.) injectionDirect intratumoral (i.t.) injection

Description

A polymer is a product formed by polymerization (addition polymerization) of a monomer. Its molecules have repeating structural units. Due to the polymer's low toxicity, stability and high transfection efficiency. Therefore, the application of polymer-based transfection systems was born. Cationic polymer-based transfection reagents can increase transfection efficiency by concentrating DNA. Exogenous DNA forms a complex with cationic polymer molecules, leading to uptake of the DNA/polymer complex by the host cell through endocytosis. Cationic polymeric transfection reagents are biocompatible, enhance stability and facilitate the successful release of engulfed genetic material from endosomes. In addition, the toxicity of the polymer can also be controlled by adjusting its structure. As earlier experiments with cationic polymers showed, polymers with short side chains are less transfectant but perform better at nuclear transcription and form stable, low-charge small complexes with nucleic acids. On the other hand, long-chain polymers showed better transfection efficiency but formed less stable complexes with high positive charges. Therefore, low toxicity and high transfection efficiency can be achieved by controlling side chain length and group modification when synthesizing polymeric vectors for in vivo transfection. To help advance in vivo experimental research, CD BioSciences has developed the POLYMER In Vivo Kit for related research.

CD BioSciences manufactures and supplies the POLYMER In Vivo Kit for in vivo studies. Contact us to find out how the POLYMER In Vivo Kit can help your work.