Kidney Cells MDCK Transfection Kit

Description

In 1958, scientists used kidney cells from an adult cocker spaniel (Canis familiaris) to create a cell line known as Madin-Darby Canine Kidney (MDCK). Immunoperoxidase staining has proven that these cells express keratin. As a typical mammalian cell line, MDCK cells have since developed into an important resource in biomedical research. They are especially helpful for researching how beta-amyloid precursor protein and its byproducts are processed. Although MDCK cells are susceptible to viral infections, they are commonly used for transfection experiments. They can provide insights into the functioning and gene expression of kidney cells, which is essential for biomedical research. Moreover, the MDCK cell line is widely utilized in influenza studies. These cells have a hyperdiploid chromosome count, showing a distribution with two distinct peaks. Most of the cells have a normal X chromosome, but there are no consistent identifiable marker chromosomes.

MDCK cells are a specific type of epithelial cells that closely resemble the polarized epithelia found in living organisms. They form monolayers that exhibit a distinct apical-basal polarity, and their apical surface is covered with microvilli. MDCK cells also develop tight junctions, which play a crucial role in maintaining the integrity of the epithelial barrier and regulating the movement of ions and solutes between cells. Thanks to their well-established polarity and tight junction formation, MDCK cells have been extensively used to investigate the mechanisms involved in epithelial transport. This includes studying the absorption and secretion of various substances, such as nutrients, electrolytes, and drugs. Researchers have also utilized MDCK cells to explore the underlying causes of diseases affecting epithelial tissues, including cystic fibrosis and influenza. Additionally, MDCK cells have proven instrumental in the production of viral vaccines, such as those for influenza and measles, due to their ability to support the replication of diverse types of viruses. Furthermore, these cells have been employed in drug discovery and development, including screening potential drug candidates for their ability to cross epithelial barriers. For researchers working with the MDCK cell line, CD BioSciences offers polymer-based transfection reagent kits.