Keratinocyte Transfection Kit

Description

Research conducted using keratinocyte cells has played a crucial role in advancing our understanding of skin biology and the progression of skin diseases. One significant finding is that exposure to ultraviolet radiation can lead to DNA damage and the formation of skin cancer by activating specific signaling pathways in keratinocyte cells. Furthermore, keratinocyte cells have been utilized to assess the effectiveness of different treatments for skin conditions, such as topical medications and gene therapy methods.

Keratinocytes are the predominant type of skin cells, accounting for nearly 95% of the epidermis. They originate in the basal layer of the skin and proliferate outward, forming dense layers within the epidermis. As outer layers are continuously shed, keratinocytes are constantly replaced. These cells are fundamental in the production of keratin, a crucial component of hair and nails. Keratinocytes are tightly interconnected, forming a protective barrier between nerves and underlying tissues in the epidermis, thereby preventing the entry of harmful substances into the body through the skin. Moreover, they play a vital role in wound healing by exhibiting a higher expression of PDGFA (fibroblast stimulating platelet-derived growth factor) than fibroblasts, thus regulating fibroblast activity.

Keratinocytes are employed in the development of laboratory techniques to assess skin irritants. They are also the primary source of the proinflammatory cytokine IL-1 in the epidermis. Studies have demonstrated that overexpression of Bcl-2 protects keratinocytes from apoptosis induced by external factors. Keratinocytes can be categorized into various subtypes, including basal keratinocytes, which are located near the basal layer. These cells have multiple applications in biomedical research. Due to their pivotal role in maintaining skin health and involvement in various skin diseases, keratinocyte cells have garnered significant attention in research. For instance, studies have revealed that abnormalities in keratinocyte differentiation and proliferation can contribute to the development of skin disorders such as psoriasis, atopic dermatitis, and skin cancer. Keratinocyte cells can be isolated from skin biopsies or obtained from commercial sources. They can be cultured and utilized as a model system to investigate diverse aspects of skin biology, including the mechanisms underlying keratinocyte differentiation and proliferation, wound healing, and the response of skin cells to environmental stressors. CD BioSciences offers advanced cationic lipid-based Keratinocyte transfection reagent kits that efficiently facilitate biomedical research concerning keratinocytes. These kits can be used to establish stable keratinocyte cell lines and achieve high transfection efficiency, as confirmed by qRT-PCR analysis.