Functional proteins refer to proteins that carry the physiological functions of the human body, and they mainly complete various metabolic activities of the human body. Functional proteins include catalytic proteins, transport proteins, immune proteins, and regulatory proteins. Catalytic protein, that is, enzyme protein, in the growth and reproduction of cells, the synthesis and decomposition of metabolites, the generation and utilization of energy, these processes all need to be completed under the catalysis of enzymes. Therefore, enzymes are a key direction of drug development; transport proteins, some proteins act as carriers and can transport specific substances to the necessary parts, so that they can complete specific functions, such proteins are called transport proteins; motor proteins, that is, the body Proteins involved in motor function, such as the main components of skeletal muscle in vertebrates are actin and myosin; immunoglobulin, the immune system of vertebrates can resist foreign invading substances, such as viruses, bacteria and other body cells , when these foreign invading substances (antigens) enter the body, they will stimulate the body's immune system to produce specific immunoglobulins (antibodies), usually each antibody has a high degree of specificity for a corresponding specific antigen , the combination of antigen and antibody forms an antigen-antibody complex, which inactivates the invading substance—antigen and excretes it from the body, thereby eliminating the interference of foreign substances on the body; hormone proteins, hormones are a type of substance produced by special cells, they pass Interaction with target cells or other organs in the system to exert their metabolic functions. In fact, many hormones themselves are proteins. Such proteins are called hormone proteins, and they have important functions in biosynthesis. Functional proteins refer to proteins that carry the physiological functions of the human body, and they mainly complete various metabolic activities of the human body. Functional proteins include catalytic proteins, transport proteins, immune proteins, and regulatory proteins. Catalytic protein, that is, enzyme protein, in the growth and reproduction of cells, the synthesis and decomposition of metabolites, the generation and utilization of energy, these processes all need to be completed under the catalysis of enzymes. Therefore, enzymes are a key direction of drug development; transport proteins, some proteins act as carriers and can transport specific substances to the necessary parts, so that they can complete specific functions, such proteins are called transport proteins; motor proteins, that is, the body Proteins involved in motor function, such as the main components of skeletal muscle in vertebrates are actin and myosin; immunoglobulin, the immune system of vertebrates can resist foreign invading substances, such as viruses, bacteria and other body cells , when these foreign invading substances (antigens) enter the body, they will stimulate the body's immune system to produce specific immunoglobulins (antibodies), usually each antibody has a high degree of specificity for a corresponding specific antigen , the combination of antigen and antibody forms an antigen-antibody complex, which inactivates the invading substance—antigen and excretes it from the body, thereby eliminating the interference of foreign substances on the body; hormone proteins, hormones are a type of substance produced by special cells, they pass Interaction with target cells or other organs in the system to exert their metabolic functions. In fact, many hormones themselves are proteins. Such proteins are called hormone proteins, and they have important functions in biosynthesis. It can be seen that functional proteins are active and very important proteins, so it is very necessary to accurately deliver these functional proteins to specific locations in the body in preclinical drug research. To this end, CD BioSciences has developed a delivery system for in vivo functional proteins using nanoparticles as carriers. The functional protein encapsulated by these carriers has good stability, long half-life in vivo, obvious therapeutic effect, and less toxic and side effects of drugs.

Figure 1. Methods for the direct delivery of proteins into cells. (Miersch S, et al.; 2016)

CD BioSciences provides customized delivery strategies, through the precise design and modification of delivery vehicles, as well as advanced technology platforms, can help you solve:

• There is no suitable method for functional protein delivery to specific tissues and organs in the body;
• Functional protein cannot easily cross cell membranes due to their size and surface chemistry
• Low payload of in vivo delivery systems
• Functional proteins are diluted and degraded during in vivo delivery without protection
• Poor stability of functional protein in vivo
• The release of functional protein in the body is uncontrolled
• Etc…

Key Features

Lipid Nanoparticles for Functional protein Delivery

• Commercial Lipids

DOGS

DODAPL, DOPE

DPPC, DOTAP, Cholesterol, PEG2k-DSPE

DOPE, DOPC, Cholesterol

• Peptide Lipids

(C14)3(L)7(NH2)8, Stearyl-R8, Cholesteryl-GALA, DSPE-R4

• Aminoglycoside Lipid

DOSP

Polymers for Functional protein Delivery

• Dendritic and multi-armed polymers

IgGMT

K- (PEG24-Ligand)-K-[K-(Stp4-C)2]2

• PEI and derivatives

PEI, pyridylthiourea- PEI

PEI

• Polyacrylic acids and polyacrylates

PPAAc

PMPC-b-PDPA, POEGMA-b-PDPA

• Polyesters and polyanhydrides

PEG-b-PCL-b-PDEA, PLGA

Poly(CPH-co-SA), poly(CPTEG-co-CPH)

• Polypeptides

PEG-pAsp(DET), pAsp(DET)

Polyarginine

• Polydisulfide
• Polyoxanorbornene

MePh-b-dG

Inorganic Nanoparticles

• Gold

AuNPs

Au-coated IONP

Au NCs

• Silica

Si NPs, core–shell RSNs, MSNs

Hypoxia-sensitive siNPs

• Carbon

OCBs

• Calcium phosphate

CaP NPs

Our Advantage:

• CD BioSciences can provide professional functional protein in vivo delivery system to achieve efficient in vivo delivery;
• Our delivery system can add modified ligands to achieve targeting;
• The in vivo functional protein delivery system has low toxicity to the body and is safe to use
• In vivo functional protein delivery system Carriers protect functional protein from dilution and degradation during in vivo delivery
• The delivery system can achieve timing, location, fixed rate and targeted release in vivo, so that experimental animals have a long-lasting effect after a single injection
• The system load is high, and the delivery requirements of different doses can be fulfilled
• Professional design and service team to provide you with reliable service and technical support feedback technical report in time

CD BioSciences is focused on developing in vivo delivery systems using our core technologies. With our high-quality products and services, your delivery effect will be greatly improved. If you can't find the perfect in vivo delivery system, you can contact us. We can provide one-to-one personal customization services.

References

1. Saeed S, et al.; Routes and barriers associated with protein and peptide drug delivery system. J Pak Med Assoc. 2021, 71(8):2032-2039.
2. Li Y, et al.; Intracellular Delivery of His-Tagged Genome-Editing Proteins Enabled by Nitrilotriacetic Acid-Containing Lipidoid Nanoparticles. Adv Healthc Mater. 2019, 8(6): e1800996.
3. Koshy ST, et al.; Injectable nanocomposite cryogels for versatile protein drug delivery. Acta Biomater. 2018, 65:36-43.
4. Yamin Li, et al.; Intracellular Functional protein Delivery Mediated by Lipids, Polymers, and Inorganic Nanomaterials for Therapeutic Applications. Adv. Therap. 2020, 2000178.
5. Meyenburg S, et al.; Fibrin encapsulated liposomes as protein delivery system. Studies on the in vitro release behavior. J Control Release. 2000, 69(1):159-68.
6. Jiskoot W, et al.; Protein instability and immunogenicity: roadblocks to clinical application of injectable protein delivery systems for sustained release. J Pharm Sci. 2012, 101(3):946-54.
7. Kumar Giri T, et al.; Nanoliposome is a Promising Carrier of Protein and Peptide Biomolecule for the Treatment of Cancer. Anticancer Agents Med Chem. 2016, 16(7):816-31.
8. Miersch S, et al.; Intracellular targeting with engineered proteins. F1000Res. 2016, 5:F1000 Faculty Rev-1947.

* For research use only. Not for use in clinical diagnosis or treatment of humans or animals.