Astrocyte Transfection Kit

Description

The human brain comprises two major cell types: neurons and glia. Neurons are responsible for transmitting nerve signals while glia safeguard neurons. Astrocytes, a sort of glial cells, make over 50% of all brain cells. In the event of any distress to the Central Nervous System (CNS), a process called gliosis is triggered. Gliosis involves the rapid differentiation of glial cells to provide mechanical support to neurons. However, excessive gliosis is symptomatic of various forms of brain cancer.

Neurons and glia are the two main cell types found in the human brain. Nerve signals are sent by neurons, while glia protect neurons. Over 50% of all brain cells are astrocytes, a kind of glial cell. Gliosis, a process, is activated whenever the Central Nervous System (CNS) is under stress. To give neurons mechanical support, glial cells undergo fast differentiation during gliosis. But severe gliosis is a sign of some types of brain cancer.

In the brain and spinal cord, glial cells called astrocytes take the form of stars. Astrocytes are supportive cells whose roles include nutrient delivery to nerve tissue, regulation of extracellular K⁺ levels, removal of neurotransmitters, and aiding in the recovery of the brain and spinal cord after traumatic injuries. Endothelial cells, which make up the blood-brain barrier, are supported biochemically by astrocytes. They are likewise reliant on the development of synapses and myelin. The intermediate filament glial fibrillary acidic protein, also known as GFAP, is expressed by many primary astrocytes. According to research, astrocytes interact with neurons via releasing gliotransmitters through a calcium ion mechanism. The central nervous system has three different types of astrocytes: fibrous, protoplasmic, and radial. The cell bodies of astrocytes are thought to be distinct and do not overlap.

In biological and gene therapy research, primary cell cultures are a useful model system that may more closely reflect the biology of healthy cells. When suitable transfection techniques are used, a large number of cultivated cell lines as well as the majority of primary cell cultures can be transfected with exogenous nucleic acids. To establish efficient transfection strategies for a particular cell type, this procedure must be optimized, particularly the methodology and chemicals to be used, as the majority of transfection methods produce severe toxicity in primary cell cultures. Compared to other options, CD BioSciences Kits for primary cells and sensitive cell lines have been developed to have much less cytotoxicity.