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SUFU Antibody
SPA-10424
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | SUFU |
| Gene Abbr. | SUFU |
| Gene ID | 51684 |
| Full Name | SUFU negative regulator of hedgehog signaling |
| Alias | JBTS32, PRO1280, SUFUH, SUFUXL |
| Introduction | SUFU (Suppressor of Fused) was identified in Drosophila as a suppressor of the Fused (Fu) kinase that is essential for Hedgehog signaling during embryonic pattern formation. SUFU suppresses Hedgehog signaling by regulating the localization of the transcription factors Gli and Ci. In Drosophila, SUFU may also positively regulate Hedgehog signaling depending on SUFU protein levels and Hedgehog signal intensity. SUFU may function as a tumor suppressor as inactivation and loss of heterozygosity of SUFU is associated with human rhabdomyosarcomas and medulloblastomas. Deletion of SUFU in mice results in embryonic lethality, while heterozygotes exhibit developmental defects characteristic of basal cell nevus syndrome. This aberrant developmental pathway is attributed to ligand-independent activation of Hedgehog signaling. GSK-3β binds and phosphorylates SUFU in vitro and additional information predicts that GSK-3β may positively regulate Hedgehog signaling through modification of SUFU. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C81H7 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ala340 of human SUFU. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala340 of human SUFU. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 54 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous level of total SUFU protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.