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SLC6A9 Knockout Cell Line
SPL-03391
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 2bp insertion |
| Target Information | |
|---|---|
| Target Name | SLC6A9 |
| Gene Abbr. | SLC6A9 |
| Gene ID | 6536 |
| Full Name | solute carrier family 6 member 9 |
| Alias | GCENSG, GLYT1 |
| Species | Human |
| Genomic Locus | chr1:44010791 |
| Transcript | NM_001024845 |
| WT Expression Level | 6.51 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | The amino acid glycine acts as an inhibitory neurotransmitter in the central nervous system. The protein encoded by this gene is one of two transporters that stop glycine signaling by removing it from the synaptic cleft. [provided by RefSeq, Jun 2016]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 2bp insertion in a coding exon of SLC6A9. |
| Description | 2bp insertion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | GAGTTTGTACTGACGAGCGT |
| PCR Primer |
Forward: CTTTATCTGGAGTGGGTCTGTGC Reverse: CTTGCTGATGGTAGCTTCTCTCC |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.