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Skp1 Antibody
SPA-10033
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Skp1 |
| Gene Abbr. | SKP1 |
| Gene ID | 6500 |
| Full Name | S-phase kinase associated protein 1 |
| Alias | EMC19, OCP-II, OCP2, SKP1A, TCEB1L |
| Introduction | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2 and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. Combinatorial interactions of different E2 and E3 proteins result in substrate specificity. Recent data suggests that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination. S phase kinase-associated protein 1 (Skp1) is a critical scaffold protein of the Skp1/CUL1/F-box (SCF) E3 ubiquitin ligase protein complex. Various F-box proteins (e.g., β-TrCP, Skp2) mediate an interaction with Skp1, via their defining and conserved domain of 40 amino acids, and with substrates to be ubiquitinated (e.g., β-catenin, p27). |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues of human Skp1. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human Skp1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IF, FC |
| Dilutions | Western Blot (1:1000); Immunofluorescence/Immunocytochemistry (1:50); Flow Cytometry (1:200) |
| MW(KDa) | 19 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | Detects endogenous levels of total Skp1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.