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Sin1/MAPKAP1 Antibody
SPA-10004
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Sin1/MAPKAP1 |
| Gene Abbr. | MAPKAP1 |
| Gene ID | 79109 |
| Full Name | MAPK associated protein 1 |
| Alias | JC310, MIP1, SIN1, SIN1b, SIN1g |
| Introduction | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTORC2 complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 470 and the C-terminus (residue 522) of human stress-activated map kinase interacting protein 1 using the numbering given in entry NP_001006618.1 (GeneID 79109). |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| Dilutions | Western Blot (1:2000-1:10000); Immunohistochemistry (1:1000-1:5000); Immunoprecipitation (2-5 μg/mg lysate) |
| MW(KDa) | 74, 78 |
| Reactivity | Human, Mouse, Rat, Bovine, Chicken, Primate, Sheep |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris-Citrate/Phosphate (pH 7.0-8.0). |
| Preservative | 0.09% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
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