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Shc Antibody
SPA-09965
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Shc |
| Gene Abbr. | SHC1 |
| Gene ID | 6464 |
| Full Name | SHC adaptor protein 1 |
| Alias | SHC, SHCA |
| Introduction | Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message. In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317. GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway. Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 725001 |
| Isotype | IgG1 |
| Immunogen | E. coli-derived recombinant human SHC1. Pro379-Val470, Trp488-Val579. P29353. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (0.5 µg/mL) |
| MW(KDa) | 46, 52, 66 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western Blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.