SGK3 Knockout Cell Line - CD BioSciences

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SGK3 Knockout Cell Line

SGK3 Knockout Cell Line

SPL-03175

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Description
1bp insertion
Target Information
Target Name SGK3
Gene Abbr. SGK3
Gene ID 23678
Full Name serum/glucocorticoid regulated kinase family member 3
Alias CISK, SGK2, SGKL
Species Human
Genomic Locus chr8:66804393
Transcript NM_013257
WT Expression Level 11.09 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 1bp insertion in a coding exon of SGK3.
Description 1bp insertion
Parental Cell Line C631
Guide RNA Sequence GGAATCTTCAGGGCCATAGC
PCR Primer Forward: ACCCACCTCACGAAGTTGTC
Reverse: ACCCACCTCACGAAGTTGTC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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