Online Inquiry
SET8 siRNA I
SPR-01036
| Size | Price |
| 1 Unit | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | SET8 |
| Gene Abbr. | KMT5A |
| Gene ID | 387893 |
| Full Name | lysine methyltransferase 5A |
| Alias | PR-Set7, PR/SET07, SET07, SET8, SETD8 |
| Introduction | SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates. SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis, and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA or siRNA results in arrest of replication forks, induction of double-stranded DNA breaks, and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53. In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase. |
| Usage | |
|---|---|
| Application | RNA interference |
| Reactivity | |
| Storage & Handling | |
|---|---|
| Storage Buffer | The siRNA product is supplied in RNAse-free water. |
| Storage Temp. | Aliquot and store at -20 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.