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Sara Antibody
SPA-09855
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Sara |
| Gene Abbr. | ZFYVE9 |
| Gene ID | 9372 |
| Full Name | zinc finger FYVE-type containing 9 |
| Alias | MADHIP, NSP, PPP1R173, SARA, SMADIP |
| Introduction | The Smad anchor for receptor activation (SARA, ZFYVE9) protein is an FYVE domain-containing protein originally identified as a regulator of TGF-β signaling. FYVE domains are zinc finger-like domains that bind to phosphatidylinositol 3-phosphate and are responsible for endosomal trafficking. While the role of Sara in TGF-β signaling has been questioned, early research studies demonstrate that Sara enhances TGF-β signaling by binding and recruiting non-activated Smad2 and Smad3 to the TGF-β receptor complex. Upon Smad2 activation, Sara dissociates from the complex while phosphorylated Smad2/3 translocates to the nucleus to bind to the common Smad, Smad4. Sara can also function as an anchor for the protein phosphatase 1 (PP1c) catalytic subunit, which is involved in the Smad7-mediated dephosphorylation of TGF-β type I receptor. Additional research studies show that expression of Sara plays a critical role in maintenance of the epithelial cell phenotype and that expression is regulated during the epithelial-to-mesenchymal transition (EMT) and fibrosis. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D5X4F |
| Immunogen | Synthetic peptide corresponding to residues surrounding Val488 of human Sara protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val488 of human Sara protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 190, 210 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total Sara protein. This antibody also recognizes a non-specific band of unknown origin at 50 kDa. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.