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ROCK1 Antibody
SPA-09697
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ROCK |
| Gene Abbr. | ROCK1 |
| Gene ID | 6093 |
| Full Name | Rho associated coiled-coil containing protein kinase 1 |
| Alias | P160ROCK, ROCK-I |
| Introduction | ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity. Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 12M03 |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to the caspase-3 cleavage site. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1-4 µg/mL) |
| MW(KDa) | 160 |
| Reactivity | Human, Mouse |
| Specificity | The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western Blotting. It does not recognize the full length protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (pH 7.2), 1% BSA, 50% Glycerol. |
| Preservative | 0.02% Thimerosal |
| Storage Temp. | Aliquot and store at -20 °C or -80 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.