Online Inquiry
ROCK1 Antibody
SPA-09686
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ROCK |
| Gene Abbr. | ROCK1 |
| Gene ID | 6093 |
| Full Name | Rho associated coiled-coil containing protein kinase 1 |
| Alias | P160ROCK, ROCK-I |
| Introduction | ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity. Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1354) of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093). |
| Usage | |
|---|---|
| Application | WB, IF, IHC, IP |
| Dilutions | Western Blot (1:5000-1:15000); Immunoprecipitation (2-5 μg/mg lysate) |
| MW(KDa) | 160 |
| Reactivity | Human, Mouse, Rat, Rabbit |
| Specificity | It is specific for human Rho Kinase Beta protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris-Citrate/Phosphate (pH 7.0-8.0). |
| Preservative | 0.09% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.