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Rictor Antibody
SPA-09601
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Rictor |
| Gene Abbr. | RICTOR |
| Gene ID | 253260 |
| Full Name | RPTOR independent companion of MTOR complex 2 |
| Alias | AVO3, PIA, hAVO3 |
| Introduction | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1650 and the C-terminus (residue 1708) of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260). |
| Usage | |
|---|---|
| Application | WB, IF, IHC, IP |
| Dilutions | Western Blot (1:2000-1:10000); Immunofluorescence (1:50-1:250); Immunohistochemistry (1:500-1:2000); Immunoprecipitation (2-5 μg/mg lysate) |
| MW(KDa) | 200 |
| Reactivity | Human, Mouse |
| Validation | Knockdown Validated. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris-Citrate/Phosphate (pH 7.0-8.0). |
| Preservative | 0.09% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.