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RhoE Antibody
SPA-09595
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | RhoE |
| Gene Abbr. | RND3 |
| Gene ID | 390 |
| Full Name | Rho family GTPase 3 |
| Alias | ARHE, Rho8, RhoE, memB |
| Introduction | Rho family small GTPases act as molecular switches that regulate processes such as cell migration, adhesion, proliferation and differentiation. Typically, they are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and are inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. Rnd1, Rnd2 and RhoE/Rnd3 comprise the evolutionarily divergent Rnd family of Rho-type small GTPases, which lack GTPase activity and therefore remain in a GTP-bound state. RhoE/Rnd3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Activity of RhoE/Rnd3 itself is regulated by ROCK1, which phosphorylates RhoE/Rnd3 at Ser11, enhancing its activity. RhoE/Rnd3 has been implicated in inhibition of DNA-damage induced and cell cycle arrest. In keratinocytes, RhoE/Rnd3 regulates differentiation through its effects on both proliferation and adhesion. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 4 |
| Immunogen | Produced by immunizing animals with Full length recombinant human RhoE. |
| Source/Purification | Antibody is produced by immunizing animals with full length recombinant human RhoE. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 29 |
| Reactivity | Human, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total RhoE protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.